ヒトiPS細胞由来腎臓オルガノイドを用いた近位尿細管モデルを開発

～薬物輸送体の機能解析と腎毒性評価のためのMicrophysiological systems (MPS)～

Summary:

We present a protocol for generating a proximal tubule-on-chip using hiPSC-derived kidney organoids. It includes organoid differentiation, proximal tubule cell isolation, and seeding on a chip, enabling enhanced transporter expression. The model supports evaluations of substrate transport by renal transporters, nephrotoxicity, and drug interactions.

本論文は、hiPSC由来の腎臓オルガノイドを用いて近位尿細管の生体模倣システム（Microphysiological systems (MPS)）を作製するプロトコルを説明したものである。このプロトコルを用いると、オルガノイドの分化誘導、近位尿細管上皮細胞の単離、チップへの播種を通してトランスポーターの発現量が向上する。このモデルを用いて、腎トランスポーターによる基質輸送能、腎毒性、薬物相互作用等を評価することができた。

Affiliations of all co-researchers:
Ryuji Yokokawa, Cheng Ma
-Department of Micro Engineering, Kyoto University, Kyoto 615-8540, Japan
Toshikazu Araoka
-Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
Minoru Takasato
-RIKEN Center for Biosystems Dynamics Research (BDR), Kobe 650-0047, Japan
-Graduate School of Medicine, Osaka University, Suita 565-0871, Japan
-Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan

Paper details:
Cheng Ma, Ramin Banan Sadeghian, Ryosuke Negoro, Kazuya Fujimoto, Toshikazu Araoka , Naoki Ishiguro, Minoru Takasato, Ryuji Yokokawa
Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transporters
STAR Protocols, Volume 6, Issue 2, 2025, 103777, ISSN 2666-1667

https://doi.org/10.1016/j.xpro.2025.103777